Essay Enzyme Carbohydrate and Biolsci 210 ‐b

Essay Enzyme Carbohydrate and Biolsci 210 †b Essay Enzyme: Carbohydrate and Biolsci 210 †b Enzymes: How can antibodies be used to treat cocaine overdoses? What cocaine? Proteins are chains of aminoisacids An alkaloid metabolite found in the leaves of Erythroxylon coca Blocks removal of dopamine from synapses in the reward pathway of the central nervous system BiolSci 210†B | 1/28/13 | 2 Cocaine is of amino our bodies by hydrolysis Proteins are chains degraded inacids Hydrolysis of cocaine yields two biologically inactive products +H2O BiolSci 210†B | 1/28/13 | 3 (Bio)chemical reactions progress through transition states (TS) Proteins are chains of amino acids BiolSci 210†B | 1/28/13 | 4 Transition chains of amino acids Proteins are states are often different from reactant and products How is cocaine different from its transition state in hydrolysis? Transition state analog (TSA) BiolSci 210†B | 1/28/13 | 5 Proteins are chains ofWhat are acids amino antibodies? Antibody (Ab) is a large Y†shaped protein produced by B†cells that is used by the immune system to identify and neutralize foreign objects Antibody recognizes a unique part of the foreign target, called an antigen Is this a tertiary or quaternary structure? BiolSci 210†B | 1/28/13 | 6 Production of chains of amino acids Proteins areantibodies can be induced by injection of specific antigens immunize with antigen obtain antibody specific for antigen BiolSci 210†B | 1/28/13 | 7 Discussion: How do antibodies help Proteins are chains of amino acidsprevent cocaine overdose? immunize with cocaine challenge with excess cocaine dies from overdose immunize with cocaine TSA challenge with excess cocaine survives 5 min BiolSci 210†B | 1/28/13 | 8 Stabilization of transition state leads Proteins are chains of amino acids to lower activation energy BiolSci 210†B | 1/28/13 | 9 Enzymes catalyzes reactions by binding to and stabilizing TS Proteins are chains of amino acids BiolSci 210†B | 1/28/13 | 10 Enzymes are highly specific Proteins are chains of amino acids catalysts Enzyme = a protein (or RNA) that catalyzes a specific chemical reaction Catalyst = enhances reaction rate without being consumed in the reaction BiolSci 210†B | 1/28/13 | 11 Proteins are chainsMost drugs inhibit enzymes of amino acids FDA orange book 1278 chemical entities approved for therapeutic use in the US 317 drugs act as enzyme inhibitors 78 target enzymes human 48 bacterial 13 viral 5 fungal 4 protozoal 1 fda.gov/cder/orange/obreadme.htm When the first print edition of Approved Drug Products with Therapeutic Equivalence Evaluations was being prepared October 1980, staff members had to choose a color for the cover. The project manager suggested, â€Å"It's almost Halloween. How about orange?† BiolSci 210†B | 1/28/13 | 12 Enzyme inhibitors are among Proteins are chains of amino acids the top 20 drugs Prescribed 1. Lipitor 2. Zocor 6. Nexium 11. Celebrex 17. Pravachol 20. Prevacid Enzyme Target HMG†CoA reductase HMG†CoA reductase Proton pump Cyclooxygenase 2 (COX†2) HMG†CoA reductase Proton pump 2004 sales $10.9 billion $5.2 billion $3.9 billion $3.3 billion $2.6 billion $2.6 billion COX†1 and COX†2 100,000 tons Over†the†counter Aspirin ~30% of approved drugs are enzyme inhibitors Understanding principles of enzyme inhibition has practical consequences BiolSci 210†B | 1/28/13 | 13 HIV of amino acids Proteins are chains maturation requires proteases HIV proteins are made as polyproteins, which are then processed by HIV protease to form individual, functional proteins BiolSci 210†B | 1/28/13 | 14 ProteinsDiscussion: How do HIV protease inhibitors function? are chains of amino acids 5 min Indinavir Amprenavir Saquinavir Ritonavir Nelfinavir BiolSci 210†B | 1/28/13 | 15